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Tiemann-Boege I, Curtis C, Shinde DN, Goodman D, Tavaré S, Arnheim N. 2009. Product length, dye choice, and detection chemistry in the bead-emulsion amplification of millions of single DNA molecules in parallel. Analytical Chemistry 81(14): 5770-6

This paper is about an ultimate high-throughput single molecule PCR method developed during my post-doctoral stay at the University of Southern California and the Johannes Kepler University. With this technology millions of single DNA molecules can be amplified in parallel on microscopic beads. It has been a turning point for my previously used technical approaches and has opened several other venues of research.

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Arnheim N., Calabrese P., Tiemann-Boege I. 2007. Mammalian hotspots of recombination. Annu Rev Genet 41:369-99

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Clark VJ, Ptak SE, Tiemann-Boege I, Qian Y, Coop G, Stone AC, Przeworski M, Arnheim N, Di Rienzo A. 2007. Combining sperm typing and LD analyses reveals differences in selective pressures or recombination rates across human populations. Genetics 175(2):795-804.

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Wyrobyk AJ, Eskenazi B, Young S, Arnheim N, Tiemann-Boege I, Jabs EW, Glaser RL, Pearson FS, Evenson D. 2006. Advancing age has differential effects of DNA damage, chromatin integrity, gene mutations, and aneuploidies in sperm. PNAS 103:9601-9606.

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Tiemann-Boege I, Calabrese P, Cochran DM, Sokol RZ, Arnheim N. 2006. High resolution recombination patterns in a region of human chromosome 21 measured by sperm typing. PLoS Genetics 2(5):e70.

In this work, we studied recombination hotspots and carried out a systematic survey of crossover rates using sperm typing during my post-doctoral stay at the University of Southern California. I developed a very efficient way of counting crossovers using real-time PCR that allowed me to count recombinants in millions of meioses. This work presented an important comparison of population genetics and sperm typing approaches and provided evidence about the possible co-regulation of hotspots of recombination. During that research, I stumbled across the intriguing properties of recombination hotspots.

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Tiemann-Boege, I., Navidi, W., Grewal, R., Cohn, D., Eskenazi, B., Wyrobek, A. J., and Arnheim, N. 2002. The observed human sperm mutation frequency cannot explain the achondroplasia paternal age effect. PNAS 99: 14952-14957

This work on the paternal age effect was the first to accurately quantify new mutations in human sperm DNA, in the exciting and unconventional work on achondroplasia. It required the development of a very sensitive method that detected a single nucleotide change in a large pool of wild type genomes. It addressed the long discussed topic whether older men have a higher risk of transmitting new mutations. This study was the first to experimentally measure the mutational load on male germcells and pointed to the novel observation of selection at the level of germcells.

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Bradley, R. D. , Martinez, R., Maltbie, M., Tiemann-Boege, I., Wichman H. A., Baker, R. J. 2003. Rapidly evolving repetitive DNA in a karyotypically megaevolved genome: factors that affect chromosomal evolution. Occasional Papers of the Museum of Texas Tech University, Number 223.

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Tiemann-Boege, I., Kilpatrick, C. W., Schmidly, D. J. and Bradley, R. D. 2000. Molecular phylogenetics of the Peromyscus boylii species group. Molecular Phylogenetics and Evolution, 16:366-378.

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Bradley, R. D. , Martinez, R., Maltbie, M., Tiemann-Boege, I., Wichman H. A., Baker, R. J. 2003. Rapidly evolving repetitive DNA in a karyotypically megaevolved genome: factors that affect chromosomal evolution. Occasional Papers of the Museum of Texas Tech University, Number 223.

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Bradley, R. D., Tiemann-Boege, I., Kilpatrick, C. W. and Schmidly, D. J. 2000. Taxonomic status of Peromyscus boylii sacarensis: inferences from DNA sequences of the mitochondrial cytochrome b gene. Journal of Mammalogy, 81:875-884.

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Clary, Melinda L., Bell, Darin M., Edwards, Cody W., Jolley, Ted W., Knyazhnitskiy, Oleksiy, Lewis-Oritt, Nicole, Mantooth, Stacy J., Peppers, Lottlie L., Tiemann-Boege, Irene, Yancey, II, Frank D., Howell, Donna J., Locke, Brian A., Baker, Robert J., and Bradley, Robert D. 1999. Checklist of mammals from twelve habitat types at Fort Bliss Military Base; 1997-1998. Occasional Papers of the Museum of Texas Tech University, Number 192.

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Bradley, Robert D. , Carroll, Darin S., Clary, Melinda L., Edwards, Cody W., Tiemann-Boege, Irene, Hamilton, Meredith J., Van Den Busche, Ronald A. and Jones, Clyde. 1999. Comments on some small mammals from the Big Bend and Trans-Pecos regions of Texas. Occasional Papers of the Museum of Texas Tech University, Number 193.

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Tiemann-Boege I, Curtis C, Shibata D, Tavare S. 2008. High-throughput analysis of methylation patterns to track cell divisions. Cellular Oncology, 30: 229-229. Conference Information: 2nd Marie Curie-Genome Architecture in Relation to Disease Meeting (MC-GARD), MAY 04-07, 2008 Madrid, SPAIN.

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Tiemann-Boege I, Curtis C, Tavare S. 2009. High throughput digital readout of methylation patterns using bead emulsion amplification. Gordon Research Conference in Human Genetics and Genomics.

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Tiemann-Boege I, Curtis C, Darryl Shibata, Tavare S. 2008. High throughput analysis of methylation patterns to track stem cell divisions. Marie Curie-- Genome architecture in relation to disease.

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Tiemann-Boege I, Calabrese P, Cochran DM, Sokol R, and Arnheim N. 2006. Analysis of fine scale recombination in a region of chromosome 21. HapMap Meeting, Cambridge, MA

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Tiemann-Boege I, Calabrese P, Cochran DM, Sokol R, and Arnheim N. 2005. Analysis of fine structure recombination patterns in a human chromosomal region not previously known to harbor a recombination hot spot. 55th Meeting of the American Society of Human Genetics. Platform Session

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Tiemann-Boege, I., Navidi W., and N. Arnheim. 2002. Mutation frequency cannot explain the achondroplasia age effect. 43rd Annual Course in Mammalian Genetics, Jackson Laboratories, Bar Harbor, ME

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Tiemann-Boege, I., C. W. Kilpatrick, D. J. Schmidly, R. D. Bradley. 1999. Molecular phylogenetics of the Peromyscus boylii species group. American Society of Mammalogists, Seattle, WA.

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Tiemann-Boege, I., , D. J. Schmidly, R. D. Bradley. 1999. Phylogenetics of the Peromyscus boylii species group using cytochrome b sequences. Texas Society of Mammalogists, Junction, Texas.

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Tiemann-Boege, I., C. W. Kilpatrick, D. J. Schmidly, R. D. Bradley. 1998. Estado taxonómico y distribución del grupo Peromyscus boylii de México. Third National Conference of mammology, Asociación Mexicana de Zoología, Xalapa, Veracruz.

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Tiemann-Boege, I., D. Alarcón-Segovia, A. Ruiz-Argüelles, J. Garcés-Eisele. 1998. Sequence specificity of a monoclonal anti-dsDNA antibody. Fifth International Conference on Systemic Lupus Erythematosus, Cancun, Mexico.